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The fed-batch fermentation method was studied by flaskculture based on the optimal medium.
然后在该培养基配方的基础上进行摇瓶分批补料发酵试验,确定最佳补料方案。
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Method: Based on enrichment culture, blood plate separation, the flaskculture and exclusive circle of methods such as oil.
方法:富集培养、血平板分离、摇瓶培养和排油圈测定。
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Objective:In this article, the shaking flaskculture conditions of strain SD59 which had a high productive capacity of lipopeptide biosurfactant have been studied.