Objective To construct recombinant cosmid vector pAxCAwt MC148 from the MC148 gene of molluscum contangiosum virus(MCV).
目的构建传染性软疣病毒（MCV ）MC14 8基因腺病毒重组粘粒。
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METHODS: Angiopoietin-1-PAxCAwt cosmid DNA and adenoviral DNA-TPC were cotransfected to 293 cells by the use of calcium phosphate precipitation method.