Methods: receptor radioligand bindingassay, receptor DNAbindingassay and immunofluorescence methods were used.
方法:采用受体放射配基结合分析、受体- DNA结合分析及免疫荧光技术。
2
The phosphorylation of STAT5 and its location were examined by double immunohistochemical staining and the binding of STAT5 with DNA was determined by electrophoretic mobility shift assay (EMSA).