Objective To construct a new retroviral vector with double-suicide genes and knockout-inducible SV40T gene, with the aim of optimizing immortalization of hepatocytes.
目的构建携带双自杀基因且可诱导敲除SV40T的逆转录病毒载体,优化目前的肝细胞永生化。
2
Since then, the suicide rate has been dropping, with much smaller increases at the end of Franklin D. Roosevelt's New Deal (1937-38), the oil crisis (1973-75) and the Double-Dip Recession (1980-82).
Conclusions the doublesuicide gene driven by KDR promoter has specific killing effect on KDR-expressing gastric tumor cells and venous endothelial cells in vitro.