The positive recombinant plasmid was identified by PCR, enzyme digestion. The nucleotide sequence of CSP3 'ending gene was determined by the dideoxychaintermination method.
阳性重组质粒经pcr、酶切鉴定，用双脱氧链末端终止法进行序列测定。
2
The result of the nucleotide sequence determined by dideoxychain-termination method indicated that 5 '-terminal nucleotide sequence of the amplified NS1 fragment was not changed.
用双脱氧链末端终止法测序结果表明，NS 1基因扩增片段5端的部分碱基序列并未发生改变。
3
The result of the nucleotide sequence determined by dideoxychain-termination method indicated that 5'-terminal nucleotide sequence of the amplified NS1 fragment was not …