Methods: the chromosome were prepared with direct method, brief cultureofcells and R-banding techniques, and the karyotypic analysis was performed on the base of complex classification.
方法：采用直接法、短期培养法和R显带技术制备染色体，根据复杂分类法进行染色体核型分析。
2
It specifically includes the isolation cultureof muscle stem cells and the construction of tissue engineered corpus spongiosum.
它具体包括肌干细胞的分离培养和组织工程海绵体的构建。
3
They then applied the substances to a cultureof human cells.