Methods:Culture CHO cells in serum-freemedia and then compare the maintainance time, morphological changes and hemagglutination titers of the cells in culture supernatant.
方法：应用无血清培养基采用小方瓶培养CHO细胞，观察细胞维持时间、培养过程的形态变化。
2
Instect cell culturemedia were optimized carefully to find new medium for special cell strain and serumfree media was manufactured commercially for suspension cell mass production;