Sandwich ELISA, dotblotting and DNA sequencing methods were used for the identification of positive clones.
通过硝酸纤维膜斑点印迹法进行阳性克隆鉴定。
2
SDS-PAGE was used to detect the purity of target protein. A higher bioactivity of purified products was shown by the determination of dot-blotting assay and fibrinogen-clotting analysis.