生物素化
Measure1. TUNEL positive cell testAdd the slice with drops of TDT and digoxin-marked dUTP reaction fluid, incubated at 37℃;then add biotinylation anti-digoxin antibody,incubated at 37℃for 30min,washed by 0.01MTBS(pH7.5) 3min for 3 times;stain with DAB,restain with hematoxylin,dehydrate,transparent and mount.
二、检测指标1、TUNEL阳性细胞检测切片滴加脱氧核糖核酸转移酶(TDT)和地高辛标记的dUTP反应液,37℃孵育120min;滴加生物素化抗地高辛抗体,37℃孵育30min,各步骤用0.01MTBS(pH 7.5)洗涤3min×3次;DAB显色,苏木素复染,脱水、透明、封片。