An amplified immunoassay with improved sensitivity has been proposed based on two-step amplification procedures of enzyme-catalyzed precipitation and biotin-streptavidin binding system (in Chapter 3).
Streptavidin has a remarkably high affinity for its natural ligand, biotin.
链霉亲和素与它的配体具有显著的高亲和力。
3
The complex and irregular structure of the biotin-binding site makes it highly optimized for biotin binding and confers great specificity to the streptavidin-biotin complexes (4).