When the denatured protein meets the gypsum, the boiling soymilk quickly coagulates. The change is so drastic that it can be see in a blink of eye in ancient days.

Objective To explore the change of corneal protein and determine if there is any new denatured protein and detect their antigenicity following alkali burn, acid burn and thermal burn.

Based on the significant difference of solvency between polysialic acid and irreversibly denatured protein, two processes removing protein from the polysialic acid broth were carried out.