Objective: To explore a method of detecting the connexin 43 (Cx43) quantity and distribution by laser scanning confocal microscope (LSCM) with a double label technique.
目的：探讨激光扫描共聚焦显微镜和荧光双重标记技术对心肌连接蛋白43进行定量和定位研究的方法。
2
Methods Double label immunofluorescence histochemical staining and confocal laser_scanning microscope were used in the experiment.
方法应用免疫荧光组织化学双标记染色技术，在激光共聚焦扫描显微镜下观察染色结果。
3
Methods: Laser scanning confocal microscope combined with immunohistochemical double labeling methods were used.