Method: Two-fold dilutionassay was used to evaluate in vitro antibacterial effect of asiaticoside on pathogenic bacteria isolated from clinic, and standard bacteria strains.
方法：采用平皿内二倍稀释法测定积雪草甙对标准菌株及临床分离致病菌株的体外抗菌活性；
2
The azotobacteria in the rhizosphere of Leymus chinensis and Agropyron mongolica were separateda, nd their activities were measured by the methods of dilution-plate method and etylene reduction assay.