The optimization of reaction conditions and the primer design are the key factors for the success accomplishment of degeneratePCR.
成功进行简并PCR的关键是设计好两组引物库和对反应条件进行优化。
2
A pair of degenerate oligonucleotide primers named L2R2were synthesized according to conserved regions of the cytadhesin proteins from M. pneumonias. MG-S6DNA were used as model in L2R2 primered PCR.