Sequence variation was screened by means of PCR single stranded conformation polymorphism (SSCP) and DNA sequencing.
用PCR -单链构象多态性(SSCP)和DNA序列分析方法确定扩增序列突变情况。
2
DNA were extracted from the peripheral blood, then TG sequence in second intron of IL - 4 was amplified by PCR, the polymorphism of that segment were then detected through 8% polyacrylamide gel.