The Phage display technology(PDT) is technology that expresses exogenic polypeptide with shell protein of phage by inserting the exogenic DNA into the shell gene.
噬菌体展示技术是一种有效的药物靶点筛选工具,可用其筛选中药的药物靶点蛋白。
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METHODS DNA from 15 clinical strains of S. aureus were extracted by phenol-chloroform method and used in the RAPD assay, phage typing were also performed.
方法对临床分离的15株金黄色葡萄球菌,酚氯仿法提取其DNA后行RAPD分型,同时进行噬菌体分型。
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Specific antibody was screened by 3 rounds of panning of phage antibody library with the fusion protein. The antigen binding activity and DNA sequences of positive clones were determined and analyzed.