Thus, the Tm of PNA–DNAduplex is barely affected by low ionic strength. This significantly facilitates the hybridization with the PNAs.
因此，PNA-DNA 的热熔温度几乎不受弱离子强度的影响，十分有利于PNA的杂交。
2
The stability of these triple helixes is so high that homopyrimidine PNA targeted to purine tracts of dsDNA invades the duplex by displacing one of the DNA strands.
Objective To investigate the value of duplex polymerase chain reaction (DPCR) in early diagnosis of Legionella pneumonia by detecting Legionella DNA in sputum and bronchoalvelar lavage fluid(BALF).