Twodimensional polyacrylamide gelelectrophoresis(2D PAGE) followed by mass spectrometry(MS) is the most widely used method of protein resolution and identification. But it exist shortcomings.
双相聚苯稀酰氨凝胶电泳(2 D PAGE)与质谱(MS)是分辨和鉴定蛋白的最常用的方法,但也存在有缺点。
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Objective To establish and optimize the two-dimensionalgelelectrophoresis technical platform for the blood serum proteome research in patients with end stage renal disease(ESRD).
目的建立和优化终末期肾病患者血清蛋白质组研究的双向电泳及相关技术,并与正常血清蛋白图谱比较。
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Methods: The contents of serum HDL subclasses in 83 CHD subjects were determined by twodimensionalgelelectrophoresis associated with immunodetection method.