Objective: To clone and express the gene of human enterokinase light chain which would be used in the cleavage and purification of fusion proteins.
研究目的：克隆表达人肠激酶轻链编码基因，以期应用于融合蛋白的切割与纯化。
2
Purification of Milli-Q water by sub-boiling distilled and employment of qualified FBS could increase the AKP staining positive level and total clone formation rate of mouse ES cells.
Results and Conclusion: the PCR screening method was convenient and fast for confirming positive recombinant clone, and there was no need for preparation and purification of the plasmid.