Hepatic stellate cell was isolated with collagenase perfusion and ladder density centrifuge. Hepatic stellate cell line IG12 was cloned with limited diluted method.
利用胶原酶灌注梯密度离心分离肝星状细胞,用有限稀释法建立大鼠肝星状细胞株ig12。
3
Conclusion HumanPD-1 gene has been cloned, recombinant retrovirus vector containing PD-1 gene constructed and L929 transfection cell line expressing PD-1 molecules selected.