We study the dissociation channel and mechanism of anisole in the experiment using the time of flight mass spectrometer with the interaction of laser.

Mechanical dissociation was used to obtain single primary spermatocytes from guinea pigs and iron channel currents in the primary spermatocytes were recorded by whole cell patch-clamp technique.

The first step of recording calcium channel current of cardiomyocytes with patch clamp technique is dissociation of myocytes.