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1 ?技术 目前，针对这三种遗传缺陷病已经建立了一些分子检测技术，如等位基因特异性PCR技术(AS-PCR)、单链构象多态性分析法(SSCP)、SNP芯片检测法等等。高分辨率熔解曲线技术(HRM)是近年兴起的一种SNP基因分型及突变检测新技术。 2 ?特异PCR N-端氨基酸微量测序技术的建立,为通过等位基因特异PCR(AS-PCR)引物设计,扩增克隆特异HMW-GS全长编码基因奠定了基础。 3 ?聚合酶链反应 根据RHD1227A等位基因序列设计等位基因特异性-聚合酶链反应(allele specific-polymerase chain reaction,AS-PCR),用于DEL表型基因分型,结果同血清学的方法作比较,探讨临床应用的可行性。 4 ?等位基因特异性 根据RHD1227A等位基因序列设计等位基因特异性-聚合酶链反应(allele specific-polymerase chain reaction,AS-PCR),用于DEL表型基因分型,结果同血清学的方法作比较,探讨临床应用的可行性。
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Methods MPLW515L and JAK2V617F mutations were simultaneously detected by alleles specific polymerase chain reaction (AS-PCR) in 190 MPD patients. 方法采用等位基因特异性聚合酶链反应(AS - PCR)及基因测序方法检测190例MPD患者的MPLW515L和JAK2V 617 F点突变。 - 2
The Maples were designed to have a small negative PCR: this was billed as one of its safety features and the reactors were licensed on that basis. 枫叶反应堆以产生少量负值pcr为设计目的：这被认为是它的安全特质之一，而反应堆也是据此而获准经营。 - 3
First his accomplishments: he invented a process called PCR, which is every bit as important to biology as the transistor is to computers. 首先看看他的成就：他发明了一项叫做聚合酶链式反应的技术，这项技术对于生物学的意义就如同晶体管对于计算机的意义。
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