PCR and Western blot were used to select the transgenic tobacco plants. ELISA was used to evaluate the expression level while colonyforming assay was used to test its biological activity.
蛋白印迹实验鉴定转基因植株，ELISA方法测定重组蛋白表达水平，集落形成实验测定重组蛋白活性。
2
In a colony-formingtest, we found that the cells lost their proliferate ability, since no colonies could be formed, if gossypol or GTW were of high concentration;