In a colony-formingtest, we found that the cells lost their proliferate ability, since no colonies could be formed, if gossypol or GTW were of high concentration;
与原位缺口翻译实验相对应,集落形成试验表明,在高浓度醋酸棉酚或GTW作用下,细胞失去繁殖的能力;
2
PCR and Western blot were used to select the transgenic tobacco plants. ELISA was used to evaluate the expression level while colonyforming assay was used to test its biological activity.