PCR and Western blot were used to select the transgenic tobacco plants. ELISA was used to evaluate the expression level while colonyformingassay was used to test its biological activity.
蛋白印迹实验鉴定转基因植株，ELISA方法测定重组蛋白表达水平，集落形成实验测定重组蛋白活性。
2
The expanded cells were further analyzed by phenotype, committed differentiation, progenitor colonyassay as well as colonyforming unite spleen.
对扩增的细胞进行免疫表型、定向分化、造血祖细胞集落分析及脾集落形成单位的研究。
3
The proliferation ability of the cells was measured by growth curve and colony-formingassay.