Affinitychromatography and ion exchange chromatography were applied in isolation and purification, and the bioactivity of expression protein was determined in cell proliferation test.
采用亲和层析和离子交换层析分离纯化，以细胞增殖实验测定表达蛋白的生物活性。
2
The GST-MBD4 fusion protein was purified from cell lysates using glutathione Sepharose 4b affinitychromatography.