The results showed that static liquid culture stimulated axillary bud development and shoot growth, but AGAR media is beneficial to induction of callus and buds formation.

Results are as follows: 1. Axillary buds culture and callus differentiation were studied in this paper, which provide experience for in vitro chromosome doubling and protoplast culture techniques.

The buds differentiation rate of callus, which induced by the axillary buds, was higher comparatively.