Objective To explore the possibility of achieving the highest resolution by using combination of anion and cationexchangechromatography.
目的：探索以阴、阳离子交换色谱串接组合为方式，进行高分辨率分离纯化蛋白质的可行性。
2
PurposeTo explore the possibility of combination of anion and cationexchangechromatography by using the polarity organic solvents in eluent.
目的研究极性有机溶剂辅助阴、阳离子交换色谱串接组合纯化蛋白质的可行性。
3
The expression supernatant was purified with cationexchangechromatography to single peak and SDS-PAGE indicated that the relative molecular mass was 6 500.