Objective To utilize tail arterial and vein catheterization technique to perform hyperinsulinemic-euglycemic clamp in conscious rats and to evaluate this technique.

Objective: Our aims were to establish the animal model of insulin resistance (IR) with high fatty diet and to evaluate it by using hyperinsulinemic euglycemic clamp technique.

Hyperinsulinemic-euglycemic clamp technique was considered to be gold standard for measurement of insulin sensitivity, and was applied further in the study of diabetes mellitus and drugs.