The positive recombinant plasmid was identified by PCR, enzyme digestion. The nucleotide sequence of CSP3 'ending gene was determined by the dideoxy chaintermination method.
阳性重组质粒经pcr、酶切鉴定，用双脱氧链末端终止法进行序列测定。
2
The coupling reaction produced 2 sulfradicals in a molecular chain of PAN with the length two times than that provided by other termination methods.
The result of the nucleotide sequence determined by dideoxy chain-termination method indicated that 5 '-terminal nucleotide sequence of the amplified NS1 fragment was not changed.