Objective: Cloning of anti CD34 single chain antibody gene.
目的：获得抗人CD34单链抗体基因。
2
To generate the recombinant antibodies, the key point is the cloning and the sequencing of the VH and VL genes from antibody secreting hybridoma cell line.
而要研制基因工程抗体，鼠源性抗体可变区基因的克隆与测序是关键。
3
A novel method in cloningantibody variable genes from immunized spleen cells was described in the report.