Positive signals were identified by observing the blue and purple spots produced by the catalytic reaction of Alkaline phosphatase to NBT and BCIP.
通过观察碱性磷酸酶对NBT和BCIP的催化反应产生蓝紫色斑点来判定阳性信号。
2
Using an NBT/BCIP color detection system, dark purple grains were seen around cells by light microscopy and indicated stable binding between DIG-WSSV and hemocytes.