The phospho-specificity of the antibody was verified by treating the membrane with (+) or without (-) calfintestinalphosphatase (CIP) after western transfer.
本抗体的磷酸化特异性验证通过小肠磷酸酶(CIP)处理或不处理western转膜结束后的膜进行。
2
Western blot analysis of extracts from A549 cells, untreated or treated with calfintestinalphosphatase (CIP), using Phospho-FAK (Tyr397) (D20B1) Rabbit mAb (upper) and FAK Antibody #3285 (lower).