Our method extended the utility of universal primers and provided implications for primer design and PCR amplification of some other genes.
新方法扩展了“通用引物”的适用范围,并为引物设计和一些其它基因的PCR放大提供了思路。
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Conclusion This highly universalprimer system in combinaition with highly specific hot initiated PCR might be used in the detection of medically important fungi in experimental or clinical specimens.