生物学

• 胰酶

  Gonadal primordial germ cells were got by isolating embryonic gonads and surrounding tissues from chicken embryos(5.5-6.5 days of incubation) and dissociating the tissues by standard trypsinization procedure(0.25%Trypin+0.02%EDTA).

  取孵化5.5～6.5天含PGCs的鸡胚生殖腺及其周围组织，经0.25％胰酶+0.02％EDTA消化后，接种于24孔培养板内。

• 胰蛋白酶消化