Other research in this area has required that cells of one type first be returned to a more generally undifferentiated stem cell state, which can introduce fresh problems.

CONCLUSION: the relatively pure and undifferentiated limbal stem cells can be obtained by combining non calcium medium and low calcium medium after the basal limbal cell tissues are cultured.

Embryonic stem cells (ES cells) are unique cell populations with the ability of self-renewal, unlimited proliferation, multipotent differentiation and maintaining their undifferentiated state.