NH2 column was used with the mobile phase of 0.1% trifluoroacetic acid-acetonitrile by gradient elution.
采用氨基色谱柱，流动相为0.1%三氟乙酸-乙腈(梯度洗脱)。
3
Preparation of TFAE through reactive distillation process had been researched, with trifluoroacetic acid and ethanol as raw materials, concentrated sulfuric acid as the catalyst.