Endothelial cells were acquired by filling collagen enzyme solution into the lumen of umbilicalveins and then cultured in M199 with 20% fetal bovine serum.
新鲜脐带内灌注胶原酶消化，获得内皮细胞，用含20%胎牛血清的M199液进行培养。
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Methods Endothelial cells were acquired by filling collagen enzyme solution into the lumen of umbilicalveins and then cultured in M199 with 20% fetal bovine serum.
方法新鲜脐带内灌注胶原酶消化，获得内皮细胞，用含20%胎牛血清的M199液进行培养。
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Conclusion: Human umbilical cord veins are good substitutes for small-caliber arterial grafts.