CD34 + cells were isolated from fresh umbilicalcordblood samples with immunomagnetic beads and seeded into the models to culture ex vivo without exogenous cytokines.
免疫磁珠分选脐血cd 34 +细胞，接种于各培养体系，无外源性细胞因子条件下进行培养。
2
AIM: to culture in vitro umbilicalcordblood mesenchymal stem cells (UCB-MSCs) by two modified methods and to observe their osteogenic differentiation potential.