The sequencing of highly conserved 5' UTR of the Chinese HGV strain has important implication for the development of genetic diagnostic assays.
HGV高度保守区5'UTR的分子克隆与核苷酸序列的测定对开展HGV感染的基因诊断具有重要意义。
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Objective; To construct a promoter identifying plasmid with GFP as reporter gene, and then identify the promoter activity of HCV 5 '- UTR with this construct.
Results it was found by sequence contrast that some mutation sites occurred mainly on UTR region, and a lot of protein sequences changed according to changes of genes coding region.