Objective: To optimize the condition of gene transienttransfection in CHO-K1 cell.
目的：以CHO - K1细胞为宿主基因瞬时转染条件的优化。
2
Methods Transient and stable transfection experiments in cells and assays of chloramphenicol acetyltransferase activity and aromatase activity were used.
方法细胞瞬时和稳定转染及氯霉素酰基转移酶和芳香族化酶活性测定。
3
The recombinant vector was transfected into HEK293 cells with liposome transfection reagents for transient expression.