In this paper sugarcane callus was cultured in vitro on the medium with smut toxin filter solution which was used as selection pressure in this experiment.
本试验采用甘蔗愈伤组织作离体培养，在培养基中加入甘蔗黑穗病粗毒素滤液为选择压力。
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The toxin-secreting culture medium of pseudomonas aeruginosa PA-103 is made in our laboratory, which is the outer dialysis solution of soybean powder digested by trypticase.