The following 4 candidate liquid media were chosen for fermentation of Sclerotinia minor, yeastextractmedium, V8 juice medium, casamino medium and soy protein medium.
The percentage of yeastextract of the nitrogen source during the growth phase and in the basal medium during the phase of induction and the time and condition that the induction starts are effective.
结果表明，基础料和补充氮源中酵母膏的比例、诱导时机和乳糖流加方式等对表达量的提高有明显的影响。
3
They produced alkali in yeastextract mannitol (YEM) medium and the generation time was more than six hours.