The expressed protein was purified by one-step affinity chromatography, and its ability of binding plasmid was investigated via gel retardation experiments.
表达产物用亲和层析一步法进行纯化，然后用凝胶阻滞试验观察重组蛋白结合质粒的能力。
2
Purpose to establish the preparation procedure of human prothrombin complex concentrate (PCC) by using two-step gel chromatography to obtain high purity and high security product.
目的应用两步凝胶色谱法制备高纯度、高安全性的人凝血酶原复合物(PCC)制剂。
3
Components of saturated alkanes in different crude oils were analyzed by gas chromatography using SE-54 chemically bonded phase capillary column and two step programmed temperature.