The lack of the sugar moiety could cause changes in the structure of the active site of the enzyme resulting in the observed modifications of the KMs.

The chemical modification of sugar moiety in nucleoside or nucleotide is always an important way to discover new lead compounds with anti-viral, anti-tumor and other biological activities.

Enzymatic hydrolysis of soybean saponin sugar-moiety produces new saponins, containing lower sugar and higher activity.