As you perform the method validation for a method, you can confirm that the retention time variation is acceptable.
只要你做了方法的验证,就可以保证保留时间变化是可接受的。
2
Remember that early peaks will be more influenced by poor equilibration than later ones. Determine if the variation in retention time is acceptable (generally, less than 0.05 min).
If the variation is acceptable and you would like a shorter total cycle time, then reduce the equilibration time, perhaps to 10 min, and repeat the experiment.