Objective To establish long-term cultured antigen-specific Tcell line and clones and to analyze their Tcellreceptor usage.
目的体外建立可长期培养的抗原特异性T细胞系及克隆,并分析其T细胞受体的使用情况。
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Tcellreceptor (TCR)gene rearrangement is an important event in Tcell ontogeny that enables T cells to specifically recognise antigens, and any dysregulation in this process may result in diseases.
In clinical practice we noticed that the successful implantation and the severity of GVHD were associated with tcell ratio of graft to receptor after pretreatment.