Aim: To develop a PCR technique for rapid screening of recombinant plasmid in subtractive library of cDNA.
目的:消减文库构建过程中,用P CR技术快速筛选重组阳性克隆。
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Methods Improved PCR-based subtractive hybridization was used to clone the genes from apoptotic prostatic carcinoma cell line DU-145 cells induced by ATRA.