Evaluate the method of determining the proliferation of human T lymphocytesubpopulations by using carboxyfluorescein diacetate succinimidyl ester labeling assay.
探讨应用活细胞荧光染料 CFSE 检测 T 淋巴细胞各亚群增殖反应的方法学。
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Objective To evaluate the method of determining the proliferation of human T lymphocytesubpopulations by using flow cytometry and carboxyfluorescein diacetate succinimidyl ester(CFSE) labeling assay.
目的探讨应用流式细胞术和活细胞荧光染料CFSE检测T淋巴细胞各亚群增殖反应的方法学。
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Methods: Peripheral t lymphocytesubpopulations were estimated by immunohistochemical method of alkaline phosphatase.