Methods: MSC were harvested from iliac and tibias crest and obtained by using gradient centrifuge and adhesiveculture methods.
方法：抽取兔髂骨及胫骨的骨髓液，经密度梯度离心法及塑料板贴壁培养法分离纯化出msc，并增殖。
2
Methods: MSC were aspirated from rabbit iliac and tibias crest and purified by gradient centrifuge and adhesiveculture methods.
方法：抽取兔髂骨及胫骨的骨髓液，采用密度梯度离心法结合贴壁培养法分离纯化出MSC，并增殖。
3
The cultivation on microcarriers (MCs) is a perfect method on the large-scale animal cell culture, which is the complete combination of adhesiveculture and suspending culture.